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From three g of cell paste about one hundred mg of homogeneous protein ended up isolated. Enzymatic action was dependent on the presence of disulfide reducing reagents which include dithiothreitol or β-mercaptoethanol through storage and purification. The indigenous molecular excess weight with the purified enzyme was estimated to be a hundred and eighty kDa dependent upon the elution quantity through a calibrated gel filtration column (information not shown).
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